Mouse anti-Human, Mouse ATM Monoclonal Antibody | anti-ATM antibody

ATM, phosphorylated (Ser1981) (Ataxia Telangiectasia Mutated, ATA, AT Complementation Group A, ATC, ATD, ATE) (MaxLight 750)

Cat. Num. AAA6247207

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Human, Mouse
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: ATM; Monoclonal Antibody; phosphorylated (Ser1981) (Ataxia Telangiectasia Mutated; ATA; AT Complementation Group A; ATC; ATD; ATE) (MaxLight 750); anti-ATM antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5H48
• Specificity: Recognizes human ATM, Mr ~370kD when phosphorylated at Ser1981. A nonspecific protein was also detected, Mr ~> 400kD. Species Crossreactivity: mouse. Sequence Homology: rat
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
• Sequence Length: 3056

• Applicable Applications for anti-ATM antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to aa1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM (KLH). The immunizing sequence has 11/12 identical amino acids in mouse and rat.
• Conjugate: MaxLight750
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Crude cell extracts (lanes 1 & 2)Crude cell extracts (lanes 1 and 2) or ATM-containing immune complexes (lanes 3 and 4) from either untreated (lanes 1 & 3) or gamma- irradiated (lanes 2 and 4) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed withMBS632147, (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system. Arrow indicates pATM (370kD).)

Product Categories/Family for anti-ATM antibody

Antibodies; Protein Kinases

References

1. Bartek, J. and J. Lukas, Nature: 421: 486-488, 2003. 2. Bakkenist, C.J. and M.B. Kastan, Nature: 421: 499-506, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 71902540
• NCBI GeneID: 472
• NCBI Accession #: NP_000042
• NCBI GenBank Nucleotide #: NP_000042.3
• UniProt Accession #: Q13315
• Molecular Weight: 370
• NCBI Official Full Name: serine-protein kinase ATM isoform a
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: ATM interactor
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated
• UniProt Entry Name: ATM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; DNA repair, damage; Kinase, protein; EC 2.7.11.1; ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasmic membrane-bound vesicle; spindle

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; 1-phosphatidylinositol-3-kinase activity; protein complex binding; protein N-terminus binding; DNA-dependent protein kinase activity; histone serine kinase activity; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; positive regulation of apoptosis; heart development; protein amino acid autophosphorylation; pre-B cell allelic exclusion; negative regulation of B cell proliferation; signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; positive regulation of neuron apoptosis; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; DNA repair; double-strand break repair via homologous recombination; neuron apoptosis; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; response to hypoxia; response to ionizing radiation; brain development; positive regulation of DNA damage response, signal transduction by p53 class mediator; response to DNA damage stimulus; oocyte development

Disease: Breast Cancer; Ataxia-telangiectasia