Mouse anti-Human, Mouse ATM Monoclonal Antibody | anti-ATM antibody

ATM, phosphorylated (Ser1981) (Ataxia Telangiectasia Mutated, ATA, AT Complementation Group A, ATC, ATD, ATE)

Cat. Num. AAA632147

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; MGC74674; DKFZp781A0353
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ATM; Monoclonal Antibody; phosphorylated (Ser1981) (Ataxia Telangiectasia Mutated; ATA; AT Complementation Group A; ATC; ATD; ATE); Anti -ATM; anti-ATM antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5H48
• Specificity: Recognizes human ATM, Mr ~370kD when phosphorylated at Ser1981. A nonspecific protein was also detected, Mr ~> 400kD. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-ATM antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence.; Dilution: Western Blot: 0.5ug/ml detected phosphorylated ATM in crude lysates from irradiated HeLa cells.; Immunoprecipitation: Phosphorylated ATM was immunoprecipitated from irradiated HeLa cells.; Immunocytochemistry: Foci are detected in irradiated human and mouse fibroblasts.
• Immunogen: Synthetic peptide corresponding to aa1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM (KLH). The immunizing sequence has 11/12 identical amino acids in mouse and rat.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Crude cell extracts (lanes 1 & 2)Crude cell extracts (lanes 1 and 2) or ATM-containing immune complexes (lanes 3 and 4) from either untreated (lanes 1 & 3) or gamma- irradiated (lanes 2 and 4) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS632147, (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system. Arrow indicates pATM (370kD).)

Related Product Information for anti-ATM antibody

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

Product Categories/Family for anti-ATM antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2304971
• NCBI GeneID: 472
• UniProt Accession #: Q13315; O15429; Q12758; Q16551; Q93007; Q9NP02; Q9UCX7; B2RNX5; Q59GH6; Q68DE6; Q6P7P1; Q8TDS0; Q96QM9
• Molecular Weight: 350,714 Da
• NCBI Official Full Name: ATM
• NCBI Official Synonym Full Names: ataxia telangiectasia mutated
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; MGC74674; DKFZp781A0353
• NCBI Protein Information: serine-protein kinase ATM; AT mutated; A-T mutated; OTTHUMP00000232981; TEL1, telomere maintenance 1, homolog
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: ATM interactor
• UniProt Gene Name: ATM
• UniProt Entry Name: ATM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq]

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; DNA repair, damage; Kinase, protein; EC 2.7.11.1; ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasmic membrane-bound vesicle; spindle

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; 1-phosphatidylinositol-3-kinase activity; protein complex binding; protein N-terminus binding; DNA-dependent protein kinase activity; histone serine kinase activity; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; positive regulation of apoptosis; heart development; protein amino acid autophosphorylation; pre-B cell allelic exclusion; negative regulation of B cell proliferation; signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; double-strand break repair; positive regulation of neuron apoptosis; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; DNA repair; double-strand break repair via homologous recombination; peptidyl-serine phosphorylation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; response to hypoxia; response to ionizing radiation; positive regulation of DNA damage response, signal transduction by p53 class mediator; brain development; response to DNA damage stimulus; oocyte development

Disease: Ataxia-telangiectasia; Breast Cancer