Human Integrin Alpha 2 (ITGa2) ELISA Kit | ITGa2 elisa kit

Human Integrin Alpha 2 (ITGa2) ELISA Kit

Cat. Num. AAA2023299

• Gene Names: ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• Reactivity: Human
• Synonyms: Integrin Alpha 2 (ITGa2); Human Integrin Alpha 2 (ITGa2) ELISA Kit; CD49b; BR; GPIa; VLA-2; VLAA2; Platelet membrane glycoprotein Ia; Alpha 2 Subunit Of VLA-2 Receptor; Very Late Activation Proteins 2 Receptor; Collagen receptor; VLA-2 alpha; ITGa2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGa2. No significant cross-reactivity or interference between ITGa2 and analogues was observed.
• Sequence Length: 1,181

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGa2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGa2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITGa2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITGa2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITGa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGa2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ITGa2 elisa kit

Signal transduction; CD & Adhesion molecule; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 116295258
• NCBI GeneID: 3673
• NCBI Accession #: NP_002194.2
• NCBI GenBank Nucleotide #: NM_002203.3
• UniProt Accession #: P17301; Q14595
• Molecular Weight: 129,295 Da
• NCBI Official Full Name: integrin alpha-2
• NCBI Official Synonym Full Names: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• NCBI Official Symbol: ITGA2
• NCBI Official Synonym Symbols: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• NCBI Protein Information: integrin alpha-2
• UniProt Protein Name: Integrin alpha-2
• Protein Family: Integrin
• UniProt Gene Name: ITGA2
• UniProt Synonym Gene Names: CD49B; GPIa
• UniProt Entry Name: ITA2_HUMAN

NCBI Description

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cell surface; focal adhesion; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane

Molecular Function: collagen binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; response to muscle activity; entry of virus into host cell; extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; cell-matrix adhesion; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of collagen binding; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to organic cyclic substance; response to L-ascorbic acid; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; substrate-bound cell migration; response to drug; integrin-mediated signaling pathway; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; detection of mechanical stimulus involved in sensory perception of pain; cell proliferation; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; positive regulation of phagocytosis, engulfment; blood coagulation; positive regulation of transmission of nerve impulse; positive regulation of inflammatory response; response to amine stimulus

Disease: Bleeding Disorder, Platelet-type, 9