Rabbit anti-Human ATG5 Polyclonal Antibody | anti-ATG5 antibody

Rabbit Anti-Human ATG5 Polyclonal

Cat. Num. AAA807521

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Human
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: ATG5; Polyclonal Antibody; Rabbit Anti-Human ATG5 Polyclonal; APG5-like Antibody; ASP Antibody; hAPG5 Antibody; ATG5 Antibody: Alkaline Phosphatase; ATG5 Antibody; anti-ATG5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-ATG5 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Synthetic peptide from the mid-protein of human ATG5
• Immunogen Species: Human
• COA: A 1:1000 dilution was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Conjugate: Alkaline Phosphatase
• Cellular Localization: Cytoplasm
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.)

Related Product Information for anti-ATG5 antibody

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).

Product Categories/Family for anti-ATG5 antibody

Cancer; Apoptosis; Autophagy

References

1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

NCBI Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; membrane; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: APG8 conjugating enzyme activity; protein binding

Biological Process: apoptosis; autophagic vacuole formation; autophagy; blood vessel remodeling; C-terminal protein lipidation; cellular response to nitrogen starvation; heart contraction; innate immune response; macroautophagy; macromitophagy; mitochondrion degradation; negative regulation of apoptosis; negative regulation of interferon type I production; negative regulation of protein ubiquitination; otolith development; post-translational protein modification; regulation of cytokine secretion during immune response; regulation of release of sequestered calcium ion into cytosol; response to drug; response to fungus; vasodilation; ventricular cardiac muscle cell development