Immunohistochemistry (IHC)
(Anti-ATF6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse ATF6 Monoclonal Antibody | anti-ATF6 antibody
Mouse Monoclonal [clone 70B1413.1] (IgG1,k) to Human ATF6
Gene Names
ATF6; ATF6A
Reactivity
Hamster, Human, Mouse, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Purified
Synonyms
ATF6; Monoclonal Antibody; Mouse Monoclonal [clone 70B1413.1] (IgG1; k) to Human ATF6; Anti-ATF6 Antibody (aa1-273; clone 70B1413.1) IHC-plus; ATF6-alpha; ATF6A; Human ATF6; anti-ATF6 antibody
Host
Mouse
Reactivity
Hamster, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
70B1413.1
Specificity
A partial protein containing amino acids 1-273 of human ATF6.
Purity/Purification
Protein G Purified
Form/Format
PBS, 0.05% BSA, 0.05% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
670
Applicable Applications for anti-ATF6 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Chromatin Immunoprecipitation
Application Notes
ChrIP, ICC, IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Recombinant human ATF6.
Immunogen Type
Recombinant protein
Immunogen
ATF6 antibody was raised against recombinant human ATF6.
Antigen Modification
aa1-273
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-ATF6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-ATF6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB)
(Western blot analysisofATF6. Lane 1, 293 cells transfected with full-length ATF6. Lane 2, 293 cells transfected with partial lengthATF6 (amino acids 1-373). Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody.)
Western Blot (WB)
(Western blot analysisofATF6. Lane 1, 293 cells transfected with full-length ATF6. Lane 2, 293 cells transfected with partial lengthATF6 (amino acids 1-373). Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody.)
Western Blot (WB)
(Western blot of ATF6in NIH3T3 cell lysate using antibody at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kD observed band; it may be an ATF6 breakdown/cleavage product.)
Western Blot (WB)
(Western blot of ATF6in NIH3T3 cell lysate using antibody at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kD observed band; it may be an ATF6 breakdown/cleavage product.)
Related Product Information for anti-ATF6 antibody
Activating Transcription Factor 6 (ATF6) is a type II transmembrane glycoprotein located on the ER membrane. In response to ER stress ATF6 is cleaved and the N-terminus including the leucine zipper (which faces the cytosol) moves to the nucleus. Once in the nucleus ATF6 binds to the ER stress response elements in ER stress genes. Site-1 and site-2 protease enzymes that process sterol regulatory binding proteins in response to cholesterol derivation cleave ATF6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,585 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-6 alpha
NCBI Official Synonym Full Names
activating transcription factor 6
NCBI Official Symbol
ATF6
NCBI Official Synonym Symbols
ATF6A
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-6 alpha
Protein Family
UniProt Gene Name
ATF6
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
UniProt Entry Name
ATF6A_HUMAN
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Product Notes
The ATF6 atf6 (Catalog #AAA245279) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 70B1413.1] (IgG1,k) to Human ATF6 reacts with Hamster, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Chromatin Immunoprecipitation. ChrIP, ICC, IHC-P (10 ug/ml), WB (1 - 3 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the ATF6 atf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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