Mouse anti-Human ATF6 Monoclonal Antibody | anti-ATF6 antibody

ATF6 Monoclonal Antibody

Cat. Num. AAA668021

• Gene Names: ATF6; ATF6A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Protein G Chromatography
• Synonyms: ATF6; Monoclonal Antibody; ATF6 Monoclonal Antibody; anti-ATF6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM1A40
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified; 25ug in 50ul/100 ug in 200ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 670

• Applicable Applications for anti-ATF6 antibody: FACS, WB
• Application Notes: Western Blot analysis: 2-4 ug/mL; FACS: 0.5 ug/10⁶ cells
• Immunogen: A partial recombinant ATF6 protein (amino acids 1-400) was used as the immunogen for this antibody
• Preparation and Storage: Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Expression analysis of ATF6. Anti- ATF6 antibody (Clone: ABM1A40) was used at 2 ug/ml on K562 lysate.)

Flow Cytometry

(Intracellular staining of full length ATF6 plasmid and blank plasmid transfected HEK293 cells. 0.5 ug of anti-ATF6 antibody was used. Green represents blank plasmid, Red represents ATF6 expression plasmid. Goat anti-mouse PE was used as secondary antibody.)

Western Blot

(Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 ug/ml on (1) Blank plasmid transfected HEK293 cells, (2) Partial length ATF6 plasmid transfected HEK293 cells and (3) Fulll length ATF6 plasmid transfected HEK293 cells.)

Related Product Information for anti-ATF6 antibody

ATF6, also named as Cyclic AMP-dependent transcription factor ATF-6 alpha is a member of bZIP family, ATF subfamily. ATF6 forms homodimer and heterodimer with ATF6-beta. ATF6 acts by activating specific unfolded protein response target genes during endoplasmic reticulum stress. ATF6 is a ubiquitously expressed transcription factor located at endoplasmic reticulum (ER) membrane. During ER stress an approximately 50kDa fragment containing the N-terminal cytoplasmic domain is cleaved by proteolysis and is translocated into the nucleus. N-glycosylation may serve as a sensor for ER homeostasis. For the binding of ATF6 to ERSE, ESRE should first bind to another transcription factor called Nuclear Transcription Factor Y (NF-Y). ATF6 could also be involved in activation of transcription by interacting with serum response factor. ATF6 is a 74 kDa protein and is highly expressed in kidney, liver, colon and mammary gland.

Product Categories/Family for anti-ATF6 antibody

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 56786157
• NCBI GeneID: 22926
• NCBI Accession #: NP_031374.2
• NCBI GenBank Nucleotide #: NM_007348.3
• UniProt Accession #: P18850; O15139; Q5VW62; Q6IPB5; Q9UEC9
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-6 alpha
• NCBI Official Synonym Full Names: activating transcription factor 6
• NCBI Official Symbol: ATF6
• NCBI Official Synonym Symbols: ATF6A
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 alpha
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF6
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
• UniProt Entry Name: ATF6A_HUMAN

NCBI Description

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Uniprot Description

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; protein folding; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction

Disease: Achromatopsia 7