Moesin (MSN) Recombinant Protein | MSN recombinant protein

Recombinant Moesin (MSN)

Cat. Num. AAA2010556

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Moesin (MSN); Recombinant Moesin (MSN); MSN recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QIEEQTKK AQQELEEQTR RALELEQERK RAQSEAEKLA KERQEAEEAK EALLQASRDQ KKTQEQLASE MAELTARVSQ LEMARKKKES EAEECHQKAQ MVQEDLEKTR AELKTAMSTP HVAEPAENEH DEQDENGAEA SAELRADAMA KDRSEEERTT EAEKNERVQK HLKALTSELA NARDESKKTT NDMIHAENMR LGRDKYKTLR QIRQGNTKQR IDEFESM
• Sequence Length: 577

• Applicable Applications for MSN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln353~Met577 (Accession # O35763) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13540689
• NCBI GeneID: 81521
• NCBI Accession #: NP_110490.1
• NCBI GenBank Nucleotide #: NM_030863.1
• UniProt Accession #: O35763
• Molecular Weight: 29.9kDa
• NCBI Official Full Name: moesin
• NCBI Official Synonym Full Names: moesin
• NCBI Official Symbol: Msn
• NCBI Protein Information: moesin; membrane-organizing extension spike protein
• UniProt Protein Name: Moesin
• UniProt Gene Name: Msn
• UniProt Entry Name: MOES_RAT

NCBI Description

involved in regulating functional associations between the cell surface and the cytoskeleton [RGD, Feb 2006]

Uniprot Description

Moesin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: extracellular space; filopodium membrane; internal side of plasma membrane; cell surface; microvillus; focal adhesion; secretory granule membrane; basolateral plasma membrane; T-tubule; cytoskeleton; extrinsic to membrane; microvillus membrane; apical part of cell; cytoplasm; apical plasma membrane; plasma membrane; uropod; nucleus; myelin sheath; vesicle; filopodium

Molecular Function: protein binding, bridging; double-stranded RNA binding; cell adhesion molecule binding; actin binding; protein kinase binding; receptor binding

Biological Process: leukocyte adhesion; membrane to membrane docking; leukocyte migration