Human Beta-arrestin-2 ELISA Kit | ARRB2 elisa kit

Human Beta-arrestin-2 ELISA Kit

Cat. Num. AAA765831

• Gene Names: ARRB2; ARB2; ARR2; BARR2
• Reactivity: Human
• Synonyms: Beta-arrestin-2; Human Beta-arrestin-2 ELISA Kit; ARRb2 (Arrestin Beta 2)/ARRB2/ARB2/ARR2/BARR2/Beta-arrestin-2/Arrestin beta-2/arrestin 3/Arrestin beta-2/arrestin; beta 2/beta-arrestin-2; ARRB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ARRB2. No significant cross-reactivity or interference between ARRB2 and analogues was observed.
• Sequence Length: 406

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ARRB2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 382545442
• NCBI GeneID: 409
• NCBI Accession #: NP_001244260.1
• NCBI GenBank Nucleotide #: NM_001257331.1
• Molecular Weight: 44,392 Da
• NCBI Official Full Name: beta-arrestin-2 isoform 6
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: ARRB2
• NCBI Official Synonym Symbols: ARB2; ARR2; BARR2
• NCBI Protein Information: beta-arrestin-2
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB2
• UniProt Synonym Gene Names: ARB2; ARR2
• UniProt Entry Name: ARRB2_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: basolateral plasma membrane; coated pit; cytoplasm; cytoplasmic vesicle; cytosol; dendritic spine; endocytic vesicle; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; D1 dopamine receptor binding; enzyme binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding

Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; blood coagulation; brain development; detection of temperature stimulus involved in sensory perception of pain; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; Notch signaling pathway; platelet activation; positive regulation of calcium ion transport; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; receptor internalization; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway