Rabbit anti-Human RNF11 Polyclonal Antibody | anti-RNF11 antibody

RNF11, ID (RNF11, RING finger protein 11)

Cat. Num. AAA6008612

• Gene Names: RNF11; CGI-123; SID1669
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RNF11; Polyclonal Antibody; ID (RNF11; RING finger protein 11); Anti -RNF11; anti-RNF11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RNF11 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: RNF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-80 amino acids from the Central region of human RNF11.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RNF11 (arrow) using rabbit polyclonal RNF11 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF11 gene.)

Immunohistochemistry (IHC)

(RNF11 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF11 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RNF11 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RNF11 antibody

The protein encoded by this gene contains a RING-H2 finger motif, which is known to be important for protein-protein interactions. The expression of this gene has been shown to be induced by mutant RET proteins (MEN2A/MEN2B). The germline mutations in RET gene are known to be responsible for the development of multiple endocrine neoplasia (MEN). [provided by RefSeq].

Product Categories/Family for anti-RNF11 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 7657520
• NCBI GeneID: 26994
• NCBI Accession #: NP_055187.1
• NCBI GenBank Nucleotide #: NM_014372.4
• UniProt Accession #: Q9Y3C5; Q5T7R8; A8KAI2
• Molecular Weight: 17,444 Da
• NCBI Official Full Name: RING finger protein 11
• NCBI Official Synonym Full Names: ring finger protein 11
• NCBI Official Symbol: RNF11
• NCBI Official Synonym Symbols: CGI-123; SID1669
• NCBI Protein Information: RING finger protein 11
• UniProt Protein Name: RING finger protein 11
• Protein Family: RING finger protein
• UniProt Gene Name: RNF11
• UniProt Entry Name: RNF11_HUMAN

NCBI Description

The protein encoded by this gene contains a RING-H2 finger motif, which is known to be important for protein-protein interactions. The expression of this gene has been shown to be induced by mutant RET proteins (MEN2A/MEN2B). The germline mutations in RET gene are known to be responsible for the development of multiple endocrine neoplasia (MEN). [provided by RefSeq, Jul 2008]

Uniprot Description

RNF11: Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: recycling endosome; early endosome; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity

Biological Process: protein autoubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process