Rabbit alpha Tubulin 4A Monoclonal Antibody | anti-TUBA4A antibody

alpha Tubulin 4A Antibody

Cat. Num. AAA9436367

• Gene Names: TUBA4A; TUBA1; H2-ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: alpha Tubulin 4A; Monoclonal Antibody; alpha Tubulin 4A Antibody; Alpha tubulin 1 antibody; Alpha-tubulin 1 antibody; FLJ30169 antibody; H2 alpha antibody; TBA4A_HUMAN antibody; Testis specific alpha tubulin antibody; Testis-specific alpha-tubulin antibody; TUBA 4A antibody; TUBA1 antibody; Tuba4a antibody; Tubulin alpha 1 (testis specific) antibody; Tubulin alpha 1 antibody; Tubulin alpha 1 chain antibody; Tubulin alpha 4a antibody; Tubulin alpha 4A chain antibody; Tubulin alpha-1 chain antibody; Tubulin alpha-4A chain antibody; Tubulin H2 alpha antibody; Tubulin H2-alpha antibody; anti-TUBA4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: JM73-24
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-TUBA4A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:200; ICC: 1:100-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1, 000 dilution. Positive control�� Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

Related Product Information for anti-TUBA4A antibody

Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta , gamma, delta and e Tubulin. alpha and beta Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple beta Tubulin isoforms (beta 1, beta 2, beta 3, beta 4, beta 5, beta 6 and beta 8) have been characterized and are expressed in mammalian tissues. beta 1 and beta 4 are present throughout the cytosol, beta 2 is present in the nuclei and nucleoplasm, and beta 3 is a neuron-specific cytoskeletal protein. gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both delta Tubulin and e Tubulin are associated with the centrosome. delta Tubulin is a homolog of the Chlamydomonas delta Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

Product Categories/Family for anti-TUBA4A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 55977476
• NCBI GeneID: 7277
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: 448
• NCBI Official Full Name: Tubulin alpha-4A chain
• NCBI Official Synonym Full Names: tubulin, alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain; tubulin H2-alpha; tubulin alpha-1 chain; tubulin, alpha 1 (testis specific)
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1
• UniProt Entry Name: TBA4A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia