Mouse anti-Human, Rat TUBA8 Monoclonal Antibody | anti-TUBA8 antibody

TUBA8 Antibody

Cat. Num. AAA9435314

• Gene Names: EHHADH; LBP; ECHD; LBFP; PBFE; L-PBE
• Reactivity: Human, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: TUBA8; Monoclonal Antibody; TUBA8 Antibody; Alpha tubulin 8 antibody; Alpha-tubulin 8 antibody; TBA8_HUMAN antibody; Tuba8 antibody; TUBAL2 antibody; Tubulin alpha 8 chain antibody; Tubulin alpha chain like 2 antibody; Tubulin alpha chain-like 2 antibody; Tubulin alpha-8 chain antibody; anti-TUBA8 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Clone Number: D7-D6
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-TUBA8 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:500; ICC: 1:100-1:500; FC/FACS: 1:100-1:200
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1, 000 dilution.)

Western Blot (WB)

(Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded �liver cancer tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

Related Product Information for anti-TUBA8 antibody

Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta , gamma, delta and e Tubulin. alpha and beta Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple beta Tubulin isoforms (beta 1, beta 2, beta 3, beta 4, beta 5, beta 6 and beta 8) have been characterized and are expressed in mammalian tissues. gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both delta Tubulin and e Tubulin are associated with the centrosome. delta Tubulin is a homolog of the Chlamydomonas delta Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

Product Categories/Family for anti-TUBA8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 261878539
• NCBI GeneID: 1962
• NCBI Accession #: NP_001159887.1
• NCBI GenBank Nucleotide #: NM_001166415.1
• UniProt Accession #: Q9NY65; Q58EZ5; A8K6Y3; B4DWG3; D3DNU0; Q08426
• Molecular Weight: 79,495 Da
• NCBI Official Full Name: peroxisomal bifunctional enzyme isoform 2
• NCBI Official Synonym Full Names: enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
• NCBI Official Symbol: EHHADH
• NCBI Official Synonym Symbols: LBP; ECHD; LBFP; PBFE; L-PBE
• NCBI Protein Information: peroxisomal bifunctional enzyme; PBE; 3,2-trans-enoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional protein, peroxisomal; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
• UniProt Protein Name: Tubulin alpha-8 chain
• UniProt Gene Name: TUBA8
• UniProt Synonym Gene Names: TUBAL2

NCBI Description

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

TUBA8: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Defects in TUBA8 are the cause of polymicrogyria with optic nerve hypoplasia (PMGONH). It is a disease characterized by extensive polymicrogyria, optic nerve hypoplasia, severe developmental delay, hypotonia, seizures, a dysplastic or absent corpus callosum and colpocephaly. Polymicrogyria is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination. Belongs to the tubulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Disease: Polymicrogyria With Optic Nerve Hypoplasia