Rabbit Akt (Thr-34) Polyclonal Antibody

Akt (Thr-34), phospho-specific

Cat. Num. AAA472002

• Reactivity: Human, Rat, Rabbit
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: Akt (Thr-34); Polyclonal Antibody; phospho-specific; PKBalpha; Protein Kinase B; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; anti-Akt (Thr-34) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Rabbit
• Clonality: Polyclonal
• Specificity: The antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of phoshorylated Akt on SDS-PAGE immunoblots of A431 + calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Akt (Thr-34) antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Western Blot: 1:1000
• Immunogen: Phospho-Akt (Thr-34) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-Akt (Thr-34) antibody

Akt, also known as Protein Kinase B (PKB) and Rac (Related to the A and C kinases), is a 60kDa serine/threonine kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4- bisphosphate which may induce the membrane translocation of Akt coincident with its phosphorylation and activation. Upon activation, Akt associates with members of the PKC family of kinases, such as PKC-delta and PKC-zeta. Ceramide-activated PKC-zeta leads to phosphorylation of the Akt-Pleckstrin Homology (PH) domain on threonine 34. This phosphorylation inhibits PIP3 binding to Akt, preventing activation of the kinase by insulin. Since Akt confers a prosurvival signal and regulates pathways in response to insulin, suppressing its activation may be one mechanism by which ceramide promotes cell death and induces insulin resistance.

References

• Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
• Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
• Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.