Rabbit IKBKG Polyclonal Antibody | anti-IKBKG antibody

IKBKG Polyclonal Antibody

Cat. Num. AAA125416

• Gene Names: IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: IKBKG; Polyclonal Antibody; IKBKG Polyclonal Antibody; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IP; IP1; IP2; IPD2; NEMO; IKKgamma; anti-IKBKG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 419

• Applicable Applications for anti-IKBKG antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human IKBKG
• Calculated Molecular Weight: 48kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IKBKG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using IKBKG antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using IKBKG antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using IKBKG antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using IKBKG antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using IKBKG antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using IKBKG antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-IKBKG antibody

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase and IKKgamma serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKbeta (Ser176 and Ser180 in IKKalpha), which causes conformational changes resulting in kinase activation (10-13).

Product Categories/Family for anti-IKBKG antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 6685695
• NCBI GeneID: 8517
• UniProt Accession #: Q9Y6K9; Q7LBY6; Q7Z7F1
• Molecular Weight: 419
• NCBI Official Full Name: NF-kappa-B essential modulator
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• NCBI Official Symbol: IKBKG
• NCBI Official Synonym Symbols: IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma
• NCBI Protein Information: NF-kappa-B essential modulator; IKKG; IKKAP1; incontinentia pigmenti; IkB kinase gamma subunit; ikB kinase subunit gamma; NFkappaB essential modulator; NF-kappa-B essential modifier; I-kappa-B kinase subunit gamma; ikB kinase-associated protein 1; inhibitor of nuclear factor kappa-B kinase subunit gamma
• UniProt Protein Name: NF-kappa-B essential modulator
• UniProt Gene Name: IKBKG
• UniProt Synonym Gene Names: FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma
• UniProt Entry Name: NEMO_HUMAN

NCBI Description

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Aug 2011]

Uniprot Description

IKKG: a regulatory subunit of the IKK-signalosome complex. Interacts preferentially with IKK-beta but also able to interact with IKK-alpha, IKAP, TAX, RIP and MAP3K14/NIK. Defects are the cause of familial incontinentia pigmenti type II (IP2).

Protein type: Protein kinase, regulatory subunit; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: spindle pole; cytoplasm; intracellular; IkappaB kinase complex; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; peroxisome proliferator activated receptor binding; protein homodimerization activity; protein heterodimerization activity; ubiquitin protein ligase binding; metal ion binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; establishment of vesicle localization; viral reproduction; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; response to virus; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to DNA damage stimulus

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 2; Ectodermal Dysplasia, Hypohidrotic, With Immune Deficiency; Immunodeficiency Without Anhidrotic Ectodermal Dysplasia; Immunodeficiency 33; Incontinentia Pigmenti; Ectodermal Dysplasia, Anhidrotic, With Immunodeficiency, Osteopetrosis, And Lymphedema