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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK4 protein (Right) or empty vector (Left) detected with AK4 antibody)

Mouse AK4 Monoclonal Antibody | anti-AK4 antibody

AK4 antibody

Gene Names
AK4; AK3; AK 4; AK3L1; AK3L2
Applications
Immunofluorescence, Western Blot
Purity
AK4 antibody was purified by affinity chromatography.
Synonyms
AK4; Monoclonal Antibody; AK4 antibody; Monoclonal AK4; Anti-AK4; Adenylate kinase 4; AK3L1; AK3L2; MGC166959; AK 4; AK-4; anti-AK4 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5B10
Purity/Purification
AK4 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
223
Applicable Applications for anti-AK4 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:2000
Biological Significance
AK4 (adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia.
Cross-Reactivity
Human
Immunogen
AK4 antibody was raised in mouse using a full length recombinant protein of human AK4 (NP_037542) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK4 protein (Right) or empty vector (Left) detected with AK4 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK4 protein (Right) or empty vector (Left) detected with AK4 antibody)
Related Product Information for anti-AK4 antibody
Mouse monoclonal AK4 antibody
Product Categories/Family for anti-AK4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
205
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,268 Da
NCBI Official Full Name
adenylate kinase 4, mitochondrial
NCBI Official Synonym Full Names
adenylate kinase 4
NCBI Official Symbol
AK4
NCBI Official Synonym Symbols
AK3; AK 4; AK3L1; AK3L2
NCBI Protein Information
adenylate kinase 4, mitochondrial
UniProt Protein Name
Adenylate kinase 4, mitochondrial
UniProt Gene Name
AK4
UniProt Synonym Gene Names
AK 4
UniProt Entry Name
KAD4_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.

Protein type: Nucleotide Metabolism - purine; Kinase, other; EC 2.7.4.6; Mitochondrial; EC 2.7.4.10

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding

Biological Process: response to drug; ATP metabolic process; nucleoside triphosphate biosynthetic process; ADP biosynthetic process; nucleoside diphosphate phosphorylation; brain development; liver development; AMP metabolic process; GTP metabolic process

Research Articles on AK4

Similar Products

Product Notes

The AK4 ak4 (Catalog #AAA533811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AK4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the AK4 ak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AK4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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