Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Islet cell antibody (ICA) Standard Curve.)
Rat Islet Cell (ICA) ELISA Kit | ICA elisa kit
Rat Islet Cell Antibody (ICA) ELISA Kit
Reactivity
Rat
Synonyms
Islet Cell (ICA); Rat Islet Cell Antibody (ICA) ELISA Kit; ICA elisa kit
Reactivity
Rat
Specificity
Rat Islet cell antibody (ICA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ICA. No significant cross-reactivity or interference between Rat ICA and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Islet cell antibody (ICA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Islet cell antibody (ICA) Standard Curve.)
Related Product Information for ICA elisa kit
Description: This Rat Islet cell antibody (ICA) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated ICA and incubated. The competitive inhibition reaction is launched between with HRP labeled ICA and unlabeled ICA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ICA in the sample. The color development is stopped and the intensity of the color is measured.
Background: an immunoglobulin that reacts with cytoplasmic components of all of the cells in the pancreatic islets. These antibodies occur in about 60% to 70% of newly identified patients with insulin-dependent diabetes mellitus, providing strong evidence for an autoimmune origin and pathogenesis of the disease. The presence of ICA can be demonstrated for years before the occurrence of symptoms. The antibody tends to disappear with time.
Background: an immunoglobulin that reacts with cytoplasmic components of all of the cells in the pancreatic islets. These antibodies occur in about 60% to 70% of newly identified patients with insulin-dependent diabetes mellitus, providing strong evidence for an autoimmune origin and pathogenesis of the disease. The presence of ICA can be demonstrated for years before the occurrence of symptoms. The antibody tends to disappear with time.
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Product Notes
The Rat ICA (Catalog #AAA9712640) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712640 ELISA Kit recognizes Rat ICA. It is sometimes possible for the material contained within the vial of "Islet Cell (ICA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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