B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) Recombinant Protein | Bcl2L2 recombinant protein

Recombinant B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2)

Cat. Num. AAA2011374

• Gene Names: BCL2L2; BCL-W
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2); Recombinant B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2); Bcl2L2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RALVADFVGY KLRQKGYVCG AGPGEGPAAD PLHQAMRAAG DEFETRFRRT FSDLAAQLHV TPGSAQQRFT QVSDELFQGG PNWGRLVAFF VFGAALCAES VNKEMEPLVGQVQEWMVAYL ETRLADWIHS SGGWAEFTAL YGDGALEEAR RLREGNWASV R
• Sequence Length: 193

• Applicable Applications for Bcl2L2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Canis familiaris; Canine (Dog)
• Expression System: Prokaryotic expression
• Residues: Arg11~Arg171 (Accession # Q45T69) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 72255565
• NCBI GeneID: 490611
• NCBI Accession #: NP_001026805.1
• NCBI GenBank Nucleotide #: NM_001031635.2
• UniProt Accession #: Q45T69
• Molecular Weight: 19.3kDa
• NCBI Official Full Name: bcl-2-like protein 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCL-W
• NCBI Protein Information: bcl-2-like protein 2; bcl2-L-2; apoptosis regulator Bcl-W
• UniProt Protein Name: Bcl-2-like protein 2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: Bcl2-L-2
• UniProt Entry Name: B2CL2_CANFA

Uniprot Description

Function: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX

By similarity.

Subunit structure: Interacts with BOP

By similarity.

Subcellular location: Mitochondrion membrane; Peripheral membrane protein

By similarity. Note: Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane

By similarity.

Domain: The BH4 motif seems to be involved in the anti-apoptotic function

By similarity.The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2

By similarity.

Sequence similarities: Belongs to the Bcl-2 family.