Goat anti-Human Allograft Inflammatory Factor-1 Polyclonal Antibody | anti-AIF1 antibody

Allograft Inflammatory Factor-1, ID (AIF1, Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

Cat. Num. AAA6001203

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Allograft Inflammatory Factor-1; Polyclonal Antibody; ID (AIF1; Allograft Inflammatory Factor 1; AIF-1; Ionized Calcium-binding Adapter Molecule 1; Protein G1; G1; IBA1); Anti -Allograft Inflammatory Factor-1; anti-AIF1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human AIF1. Species sequence homology: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-AIF1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 1:32,000 & 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-NKQFLDDPKYSSDED from an internal region of human AIF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta (formalin-fixed, paraffin-embedded) stained with AIF1 antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Chemiluminescence

(Antibody (1ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-AIF1 antibody

AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.

Product Categories/Family for anti-AIF1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1229022
• NCBI GeneID: 199
• UniProt Accession #: P55008; O43904; Q9UIV4; Q9UKS9; A8K406
• Molecular Weight: 16,703 Da
• NCBI Official Full Name: allograft inflammatory factor-1
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; phagocytosis, engulfment; response to electrical stimulus; inflammatory response; negative regulation of apoptosis