Typical Testing Data/Standard Curve (for reference only)
General HIS ELISA Kit | HIS elisa kit
HIS (Histamine) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pigTIMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pigTIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pigTIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Guinea pigTIMP-2. You can calculate the concentration of Guinea pigTIMP-2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
HAL: Defects in HAL are the cause of histidinemia (HISTID). It is an autosomal recessive disease characterized by increased histidine and histamine as well as decreased urocanic acid in body fluids. Belongs to the PAL/histidase family.
Protein type: EC 4.3.1.3; Lyase; Energy Metabolism - nitrogen; Amino Acid Metabolism - histidine
Chromosomal Location of Human Ortholog: 12q22-q24.1
Cellular Component: cytosol
Molecular Function: histidine ammonia-lyase activity
Biological Process: histidine catabolic process to glutamate and formamide; biosynthetic process; histidine catabolic process; histidine catabolic process to glutamate and formate
Disease: Histidinemia
Research Articles on HIS
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Product Notes
The General HIS hal (Catalog #AAA2515154) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515154 ELISA Kit recognizes General HIS. It is sometimes possible for the material contained within the vial of "HIS, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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