Rat AID Monoclonal Antibody | anti-AID antibody

RAT ANTI HUMAN AID

Cat. Num. AAA219435

• Gene Names: AICDA; AID; ARP2; CDA2; HIGM2; HEL-S-284
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: AID; Monoclonal Antibody; RAT ANTI HUMAN AID; anti-AID antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: EK2-5G9
• Specificity: This item specifically recognizes human AID (Activation-induced cytidine deaminase), a 24kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination (CSR), somatic hypermutation (SHM) and gene conversion (GC). Studies suggest that AID is selectively expressed by germinal centre (GC) B cells and B cell-derived lymphomas, and that a deficiency in AID is responsible for the autosomal recessive form of the immunodeficient condition Hyper-IgM syndrome.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 188

• Applicable Applications for anti-AID antibody: ELISA (EIA), Flow cytometry (FC/FACS)*, Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.; Immunohistology - Paraffin: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Synthetic peptide corresponding to amino acids 185-198 of human AID.; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-AID antibody

MBS219435 specifically recognizes human AID (Activation-induced cytidine deaminase), a 24kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination (CSR), somatic hypermutation (SHM) and gene conversion (GC). Studies suggest that AID is selectively expressed by germinal centre (GC) B cells and B cell-derived lymphomas, and that a deficiency in AID is responsible for the autosomal recessive form of the immunodeficient condition Hyper-IgM syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 10190700
• NCBI GeneID: 57379
• NCBI Accession #: NP_065712.1
• NCBI GenBank Nucleotide #: NM_020661.2
• UniProt Accession #: Q9GZX7; Q6QJ81; Q8NFC1
• Molecular Weight: 22,614 Da
• NCBI Official Full Name: single-stranded DNA cytosine deaminase
• NCBI Official Synonym Full Names: activation-induced cytidine deaminase
• NCBI Official Symbol: AICDA
• NCBI Official Synonym Symbols: AID; ARP2; CDA2; HIGM2; HEL-S-284
• NCBI Protein Information: single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos
• UniProt Protein Name: Single-stranded DNA cytosine deaminase
• Protein Family: AIDA-I autotransporter
• UniProt Gene Name: AICDA
• UniProt Synonym Gene Names: AID
• UniProt Entry Name: AICDA_HUMAN

NCBI Description

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

Uniprot Description

AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.38; Hydrolase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2