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Quality Control (Western blot analysis of immunized recombinant protein, using anti-AHNAK monoclonal antibody. )

Mouse anti-Human AHNAK Monoclonal Antibody | anti-AHNAK antibody

Mouse monoclonal antibody Anti-Human AHNAK

Gene Names
AHNAK; AHNAKRS
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry
Synonyms
AHNAK; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human AHNAK; Homo sapiens AHNAK nucleoprotein (desmoyokin) (AHNAK); transcript variant 1; AHNAKRS; MGC5395; anti-AHNAK antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2014C3a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
18836
Applicable Applications for anti-AHNAK antibody
Dot Blot (DB), Immunocytochemistry (ICC)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-AHNAK monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-AHNAK monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-AHNAK mAb. [Lot No. 2014C3a-1] )

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-AHNAK mAb. [Lot No. 2014C3a-1] )
Related Product Information for anti-AHNAK antibody
May be required for neuronal cell differentiation. [UniProtKB Function]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,061 Da
NCBI Official Full Name
Homo sapiens AHNAK nucleoprotein (AHNAK), transcript variant 1, mRNA
NCBI Official Synonym Full Names
AHNAK nucleoprotein
NCBI Official Symbol
AHNAK
NCBI Official Synonym Symbols
AHNAKRS
NCBI Protein Information
neuroblast differentiation-associated protein AHNAK; AHNAK-related; desmoyokin
UniProt Protein Name
Neuroblast differentiation-associated protein AHNAK
UniProt Gene Name
AHNAK
UniProt Entry Name
AHNK_HUMAN

Uniprot Description

AHNAK: a giant propeller-like protein that associates with calcium channel proteins of cardiomyocytes and other cells. Translocates from the cytosol to the plasma membrane during the formation of cell-cell contacts and the development of epithelial polarity. At the plasma membrane, it associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The AHNAK/annexin 2/S100A10 complex regulates cortical actin cytoskeleton organization and the architecture of the cell membrane.

Protein type: Actin-binding; Adaptor/scaffold; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: actin cytoskeleton; cell-cell adherens junction; costamere; cytoplasm; cytosol; focal adhesion; lipid raft; lysosomal membrane; membrane; nucleus; plasma membrane; sarcolemma; vesicle

Molecular Function: protein binding

Biological Process: protein oligomerization; regulation of RNA splicing

Research Articles on AHNAK

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Product Notes

The AHNAK ahnak (Catalog #AAA120134) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human AHNAK reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AHNAK can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the AHNAK ahnak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHNAK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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