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Typical Testing Data/Standard Curve (for reference only)

Mouse Syndecan-2 ELISA Kit | Sdc2 elisa kit

Mouse Syndecan-2 ELISA Kit

Gene Names
SDC2; HSPG; CD362; HSPG1; SYND2
Reactivity
Mouse
Synonyms
Syndecan-2; Mouse Syndecan-2 ELISA Kit; Sdc2/HSPG/CD362/Fibroglycan/HSPG1/SDC2/SYND2/CD362 antigen/Fibroglycan/fibroglycan/heparan sulfate proteoglycan 1; cell surface-associated/Heparan sulfate proteoglycan core protein/HSPG/HSPG1syndecan proteoglycan 2/SYND2cell surface-associated heparan sulfate proteoglycan 1/syndecan 2/syndecan-2; Sdc2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of SDC1. No significant cross-reactivity or interference between SDC1 and analogues was observed.
Sequence Length
201
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SDC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SDC1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Sdc2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SDC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SDC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SDC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SDC1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,160 Da
NCBI Official Full Name
syndecan-2
NCBI Official Synonym Full Names
syndecan 2
NCBI Official Symbol
SDC2
NCBI Official Synonym Symbols
HSPG; CD362; HSPG1; SYND2
NCBI Protein Information
syndecan-2
UniProt Protein Name
Syndecan-2
Protein Family
UniProt Gene Name
SDC2
UniProt Synonym Gene Names
HSPG1; SYND2; HSPG
UniProt Entry Name
SDC2_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-2 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-2 expression has been detected in several different tumor types. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-2: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Preferentially expressed in cells of mesenchymal origin. Is tyrosine phosphorylated and forms a complex with EphB2 in mouse brain. Plays a role in mediating adhesion and proliferation of colon carcinoma cells.

Protein type: Cell surface; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q22-q23

Cellular Component: cell soma; cell surface; Golgi lumen; integral to membrane; lysosomal lumen; plasma membrane; synapse

Molecular Function: PDZ domain binding; protein binding

Biological Process: axon guidance; bioluminescence; carbohydrate metabolic process; cell migration; chondroitin sulfate metabolic process; dendrite morphogenesis; ephrin receptor signaling pathway; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; phototransduction, visible light; regulation of dendrite morphogenesis; response to caffeine; response to hypoxia; retinoid metabolic process; vitamin metabolic process; wound healing

Research Articles on Sdc2

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Product Notes

The Mouse Sdc2 sdc2 (Catalog #AAA761063) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA761063 ELISA Kit recognizes Mouse Sdc2. It is sometimes possible for the material contained within the vial of "Syndecan-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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