Human C-Type Lectin Domain Family 7 ELISA Kit | CLEC7A elisa kit

Human C-Type Lectin Domain Family 7, Member A (CLEC7A) ELISA Kit

Cat. Num. AAA2031899

• Gene Names: CLEC7A; BGR; CD369; CANDF4; DECTIN1; CLECSF12
• Reactivity: Human
• Synonyms: C-Type Lectin Domain Family 7; Human C-Type Lectin Domain Family 7; Member A (CLEC7A) ELISA Kit; BGR; CLECSF1; CANDF4; CLECSF12; DECTIN1; Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; CLEC7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 7, Member A (CLEC7A).; No significant cross-reactivity or interference between C-Type Lectin Domain Family 7, Member A (CLEC7A) and analogues was observed.
• Sequence Length: 201

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for CLEC7A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 7, Member A (CLEC7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 7, Member A (CLEC7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CLEC7A elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 13384604
• NCBI GeneID: 64581
• NCBI Accession #: NP_072092.2
• NCBI GenBank Nucleotide #: NM_022570.4
• Molecular Weight: 13,138 Da
• NCBI Official Full Name: C-type lectin domain family 7 member A isoform b
• NCBI Official Synonym Full Names: C-type lectin domain family 7 member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CD369; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A
• UniProt Protein Name: C-type lectin domain family 7 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; integral to membrane; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: carbohydrate binding; metal ion binding; MHC protein binding; pattern recognition receptor activity; protein binding

Biological Process: carbohydrate mediated signaling; cell recognition; defense response to protozoan; inflammatory response; innate immune response; leukocyte activation during immune response; pattern recognition receptor signaling pathway; phagocytosis, recognition; stimulatory C-type lectin receptor signaling pathway; T cell activation

Disease: Aspergillosis, Susceptibility To; Candidiasis, Familial, 4