Rabbit anti-Human RSK2 Polyclonal Antibody | anti-RSK2 antibody

RSK2, Phosphorylated (Ser369) (Ribosomal S6 Kinase 2, RSK2, RSK-2, p90-RSK2, pp90RSK2, 90kD Ribosomal Protein S6 Kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated Protein Kinase 1, ISPK1, ISPK-1, MAP Kinase-activated Protein Kinase 1b, MAPK-activated Prote

Cat. Num. AAA6498278

• Gene Names: RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RSK2; Polyclonal Antibody; Phosphorylated (Ser369) (Ribosomal S6 Kinase 2; RSK-2; p90-RSK2; pp90RSK2; 90kD Ribosomal Protein S6 Kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated Protein Kinase 1; ISPK1; ISPK-1; MAP Kinase-activated Protein Kinase 1b; MAPK-activated Prote; Ribosomal Protein S6 Kinase 90kDa Polypeptide 3; CLS; EC=2.7.11.1; HU-3; MRX19; RSK; anti-RSK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS6KA3 when phosphorylated at Ser369.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RSK2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser369 of human RPS6KA3 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-RPS6KA3-pS369 Phospho-specific Pab (RB13311) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-RSK2 antibody

Antibodies; Protein Kinases; Ribosomal

References

1. Yang, X., et al., Cell 117(3):387-398 (2004). 2. Guimiot, F., et al., Gene Expr. Patterns 4(1):111-114 (2004). 3. Zeniou, M., et al., (er) Nucleic Acids Res. 32(3):1214-1223 (2004). 4. Vaidyanathan, H., et al., J. Biol. Chem. 278(34):32367-32372 (2003). 5. Zhang, Y., et al., J. Biol. Chem. 278(15):12650-12659 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4759050
• NCBI GeneID: 6197
• NCBI Accession #: NP_004577
• NCBI GenBank Nucleotide #: NM_004586.2
• UniProt Accession #: P51812; Q4VAP3; Q59H26; Q5JPK8; Q7Z3Z7; B2R9V4
• Molecular Weight: 83,736 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• NCBI Official Symbol: RPS6KA3
• NCBI Official Synonym Symbols: CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• UniProt Gene Name: RPS6KA3
• UniProt Synonym Gene Names: ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
• UniProt Entry Name: KS6A3_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]