Mouse Actin Monoclonal Antibody | anti-ACTA2 antibody

Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438388

• Gene Names: ACTA2; AAT6; ACTSA; MYMY5
• Reactivity: Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Dog, Rabbit, Mouse, Rat, Guinea Pig, Chicken
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Actin; Monoclonal Antibody; Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody; ACTA2; Actin Alpha 2 Smooth Muscle Aorta; Actin Aortic Smooth Muscle; Actin Vascular Smooth Muscle; ACTSA; ACTVS; Alpha 2 Actin; Alpha Actin 2; Alpha Cardiac Actin; Alpha Smooth Muscle; Alpha-actin-2; Aortic Smooth Muscle; ASMA; Cell Growth-inhibiting Gene 46 Protein; Growth Inhibiting Gene 46; anti-ACTA2 antibody

• Host: Mouse
• Reactivity: Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Dog, Rabbit, Mouse, Rat, Guinea Pig, Chicken
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: SPM332
• Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 377

• Applicable Applications for anti-ACTA2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
• Hu-Chromosome Location: 10q23.31
• Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (SPM332).)

References

Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96

NCBI and Uniprot Product Information

• NCBI GI #: 213688375
• NCBI GeneID: 59
• NCBI Accession #: NP_001135417.1
• NCBI GenBank Nucleotide #: NM_001141945.2
• UniProt Accession #: P62736; P03996; P04108; Q6FI19; B2R8A4
• Molecular Weight: 42kDa
• NCBI Official Full Name: actin, aortic smooth muscle
• NCBI Official Synonym Full Names: actin, alpha 2, smooth muscle, aorta
• NCBI Official Symbol: ACTA2
• NCBI Official Synonym Symbols: AAT6; ACTSA; MYMY5
• NCBI Protein Information: actin, aortic smooth muscle
• UniProt Protein Name: Actin, aortic smooth muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA2
• UniProt Synonym Gene Names: ACTSA; ACTVS
• UniProt Entry Name: ACTA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Uniprot Description

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Contractile

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium; protein complex

Molecular Function: protein kinase binding

Biological Process: muscle contraction; response to virus

Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome