Rabbit E2F2 Polyclonal Antibody | anti-E2F2 antibody

E2F2 Polyclonal Antibody

Cat. Num. AAA3012622

• Gene Names: E2F2; E2F-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: E2F2; Polyclonal Antibody; E2F2 Polyclonal Antibody; E2F-2; dE2F2; E2F transcription factor 2; Transcription factor E2F2; anti-E2F2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: E2F2 polyclonal antibody detects endogenous levels of E2F2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 437

• Applicable Applications for anti-E2F2 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:2,000; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of E2F2 on different lysates using anti-E2F2 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: 293T Lane 3: HepG2)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells with E2F2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Related Product Information for anti-E2F2 antibody

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

NCBI and Uniprot Product Information

• NCBI GI #: 4758226
• NCBI GeneID: 1870
• NCBI Accession #: NP_004082.1
• NCBI GenBank Nucleotide #: NP_004082.1
• UniProt Accession #: Q14209; P56931
• Molecular Weight: 48kDa
• NCBI Official Full Name: transcription factor E2F2
• NCBI Official Synonym Full Names: E2F transcription factor 2
• NCBI Official Symbol: E2F2
• NCBI Official Synonym Symbols: E2F-2
• NCBI Protein Information: transcription factor E2F2
• UniProt Protein Name: Transcription factor E2F2
• Protein Family: Transcription factor
• UniProt Gene Name: E2F2
• UniProt Synonym Gene Names: E2F-2
• UniProt Entry Name: E2F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F2: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of cell cycle; mitotic cell cycle