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Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)
Mouse anti-Human, Mouse ACTB Monoclonal Antibody | anti-ACTB antibody
ACTB (Actin, Cytoplasmic 1, Beta-actin) (FITC)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ACTB; Monoclonal Antibody; ACTB (Actin; Cytoplasmic 1; Beta-actin) (FITC); anti-ACTB antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G4-F9
Specificity
Recognizes human ACTB. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-ACTB antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-375 from human ACTB (AAH01301) with GST tag. MW of the GST tag alone is 26kD.
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)
Western Blot (WB)
(Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)
Western Blot (WB)
(ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)
Product Categories/Family for anti-ACTB antibody
References
1. Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis. Yu Y, Shen H, Yu H, Zhong F, Zhang Y, Zhang C, Zhao J, Li H, Chen J, Liu Y, Yang P.Mol Biosyst. 2011 Jun;7(6):1908-16. Epub 2011 Apr 6. 2. Identification of early transcripts related to male development in chicken embryos. Lin YP, Chen LR, Chen CF, Liou JF, Chen YL, Yang JR, Shiue YL.Theriogenology. 2010 Aug 20. 3. Anti-microtubule 'plinabulin' chemical probe KPU-244-B3 labeled both alpha- and beta-tubulin. Yamazaki Y, Sumikura M, Hidaka K, Yasui H, Kiso Y, Yakushiji F, Hayashi Y.Bioorg Med Chem. 2010 Mar 23. 4. 7beta-Hydroxy-epiandrosterone-mediated regulation of the prostaglandin synthesis pathway in human peripheral blood monocytes. Mee SL, Hennebert O, Ferrec C, Wulfert E, Morfin R.Steroids. 2008 Oct;73(11):1148-59. Epub 2008 May 14.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
Research Articles on ACTB
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Product Notes
The ACTB (Catalog #AAA6145762) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACTB (Actin, Cytoplasmic 1, Beta-actin) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACTB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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