Mouse anti-Human ACBD6 Monoclonal Antibody | anti-ACBD6 antibody

Anti-ACBD6 Antibody, Mouse Monoclonal

Cat. Num. AAA8104898

• Reactivity: Human
• Applications: Western Blot, ELISA, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: ACBD6; Monoclonal Antibody; Anti-ACBD6 Antibody; Mouse Monoclonal; ACBD6 Antibody; Mouse MAb; acyl-CoA binding domain containing 6; anti-ACBD6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 4A9D5E9
• Specificity: Human ACBD6.; No cross-reactivity in ELISA with Insect cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACBD6 antibody: Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000; IP: 1-2uL/mg of lysate; ELISA (Det): 1:5000-1:50,000; ELISA (Det): This antibody will detect Human ACBD6 in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Human ACBD6 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACBD6 (rh ACBD6). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ACBD6 was immunoprecipitated using:Lane A:0.5 mg hela Whole Cell Lysate0.5 uL anti-ACBD6 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ACBD6 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)

Western Blot (WB)

(Anti-ACBD6 mouse monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDaObserved band size:35 kDa)

Related Product Information for anti-ACBD6 antibody

Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.

References

Soupene E, et al. (2008) Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells. J Lipid Res. 49(5): 1103-12.

NCBI and Uniprot Product Information

• NCBI GI #: 14150169
• NCBI GeneID: 84320
• NCBI Accession #: NP_115736.1
• NCBI GenBank Nucleotide #: NM_032360.3
• UniProt Accession #: Q9BR61
• Molecular Weight: 31,151 Da
• NCBI Official Full Name: acyl-CoA-binding domain-containing protein 6
• NCBI Official Synonym Full Names: acyl-CoA binding domain containing 6
• NCBI Official Symbol: ACBD6
• NCBI Protein Information: acyl-CoA-binding domain-containing protein 6; acyl-Coenzyme A binding domain containing 6
• UniProt Protein Name: Acyl-CoA-binding domain-containing protein 6
• Protein Family: Acyl-CoA-binding domain-containing protein
• UniProt Gene Name: ACBD6
• UniProt Entry Name: ACBD6_HUMAN

Uniprot Description

ACBD6: Binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1-CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0- CoA. Does not bind fatty acids.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cytoplasm

Molecular Function: acyl-CoA binding; lipid binding