<
Immunohistochemistry (IHC)
(Immunochemical staining of rat IFNA5 in rat lymph node with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat interferon-alpha/IFNA5 Polyclonal Antibody | anti-IFNA5 antibody
Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
interferon-alpha/IFNA5; Polyclonal Antibody; Anti-interferon-alpha/IFNA5 Antibody; Rabbit Polyclonal; IFNA5/IFNaG Antibody; Rabbit PAb; Antigen Affinity Purified; interferon; alpha 5; Anti-IFNA5 Antibody; anti-IFNA5 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat IFNA5/IFNaG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-IFNA5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:2500-1:10,000
IP: 1-2uL/mg of lysate
ELISA: 1:25,000-1:50,000
IHC-P: 1:2500-1:10,000
IP: 1-2uL/mg of lysate
Immunogen
Recombinant Rat IFNA5/IFNaG protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat IFNA5/IFNaG. IFNA5/IFNaG specific IgG was purified by Rat IFNA5/IFNaG affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat IFNA5 in rat lymph node with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat IFNA5 in rat lymph node with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat IFNA5 in rat thymus with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat IFNA5 in rat thymus with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(rat IFNA5 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-rat IFNA5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat IFNA5 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Immunoprecipitation (IP)
(rat IFNA5 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-rat IFNA5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat IFNA5 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Western Blot (WB)
(Anti-IFNA5 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-IFNA5 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-IFNA5 antibody
Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
References
Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The IFNA5 (Catalog #AAA8112822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's interferon-alpha/IFNA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:2500-1:10,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the IFNA5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "interferon-alpha/IFNA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
