ACBD6 recombinant protein

ACBD6 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120689

• Purity: >96% as determined by SDS-PAGE
• Synonyms: ACBD6; ACBD6 Protein; Human; Recombinant (His Tag); Human ACBD6 Protein (His Tag); acyl-CoA binding domain containing 6; ACBD6 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala282

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the full length of human ACBD6 (NP_115736.1)(Met 1-Ala 282) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ACBD6 recombinant protein

Background: Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.

References

Soupene E, et al. (2008) Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells. J Lipid Res. 49(5): 1103-12.

NCBI and Uniprot Product Information

• NCBI GI #: 14150169
• NCBI GeneID: 84320
• NCBI Accession #: NP_115736.1
• NCBI GenBank Nucleotide #: NM_032360.3
• UniProt Accession #: Q9BR61
• Molecular Weight: 31,151 Da
• NCBI Official Full Name: acyl-CoA-binding domain-containing protein 6
• NCBI Official Synonym Full Names: acyl-CoA binding domain containing 6
• NCBI Official Symbol: ACBD6
• NCBI Protein Information: acyl-CoA-binding domain-containing protein 6; acyl-Coenzyme A binding domain containing 6
• UniProt Protein Name: Acyl-CoA-binding domain-containing protein 6
• Protein Family: Acyl-CoA-binding domain-containing protein
• UniProt Gene Name: ACBD6
• UniProt Entry Name: ACBD6_HUMAN

Uniprot Description

ACBD6: Binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1-CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0- CoA. Does not bind fatty acids.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cytoplasm

Molecular Function: acyl-CoA binding; lipid binding