Mouse anti-Human NAA10/ARD1 Monoclonal Antibody | anti-NAA10 antibody

Anti-NAA10/ARD1 Antibody, Mouse Monoclonal

Cat. Num. AAA8107304

• Gene Names: NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: NAA10/ARD1; Monoclonal Antibody; Anti-NAA10/ARD1 Antibody; Mouse Monoclonal; ARD1 Antibody; Mouse MAb; N(alpha)-acetyltransferase 10; NatA catalytic subunit; Anti-ARD1 Antibody; Anti-ARD1A Antibody; Anti-ARD1P Antibody; Anti-DXS707 Antibody; Anti-MCOPS1 Antibody; Anti-NAA10 Antibody; Anti-NATD Antibody; Anti-OGDNS Antibody; Anti-TE2 Antibody; anti-NAA10 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 10
• Specificity: Human ARD1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NAA10 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human ARD1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ARD1A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-ARD1A mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ARD1A mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 26 kDa)

Western Blot (WB)

(Anti-ARD1A mouse monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:33 kDa)

Related Product Information for anti-NAA10 antibody

ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

References

Tribioli C., et al., (1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

NCBI and Uniprot Product Information

• NCBI GI #: 728880
• NCBI GeneID: 8260
• UniProt Accession #: P41227; A6NM98
• Molecular Weight: 235
• NCBI Official Full Name: N-alpha-acetyltransferase 10
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 10, NatA catalytic subunit
• NCBI Official Symbol: NAA10
• NCBI Official Synonym Symbols: TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• NCBI Protein Information: N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A
• UniProt Protein Name: N-alpha-acetyltransferase 10
• Protein Family: N-alpha-acetyltransferase
• UniProt Gene Name: NAA10
• UniProt Synonym Gene Names: ARD1; ARD1A; TE2
• UniProt Entry Name: NAA10_HUMAN

NCBI Description

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily.

Protein type: Amino Acid Metabolism - tyrosine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; EC 2.3.1.88; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: membrane; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function: peptide alpha-N-acetyltransferase activity; N-acetyltransferase activity; protein binding; acetyltransferase activity; ribosome binding

Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; internal protein amino acid acetylation; N-terminal peptidyl-serine acetylation; DNA packaging

Disease: Ogden Syndrome; Microphthalmia, Syndromic 1