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Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:RPE)
Rat anti-Mouse ABCA1 Monoclonal Antibody | anti-ABCA1 antibody
ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1, ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD) (MaxLight 550)
Gene Names
Abca1; Abc1; ABC-1
Reactivity
Mouse
Applications
Flow Cytometry, Immunofluorescence
Purity
Purified by Protein G affinity chromatography.
Synonyms
ABCA1; Monoclonal Antibody; ABCA1 (ABC 1; ABC Transporter 1; ATP-binding Cassette 1; ATP-binding Cassette Transporter 1; CERP; Cholesterol Efflux Regulatory Protein; HDLDT1; TGD) (MaxLight 550); anti-ABCA1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5A1-1422
Specificity
Recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1).
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLightTM550.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-ABCA1 antibody
Flow Cytometry (FC), Immunofluorescence (IF)
Application Notes
Suitable for use in Flow Cytometry and Immunofluorescence.
Not recommended for use in Western Blot.
Other applications not tested. Optimal dilutionsto be determined by the researcher.
Note: Applications are based on unconjugated antibody.
Not recommended for use in Western Blot.
Other applications not tested. Optimal dilutionsto be determined by the researcher.
Note: Applications are based on unconjugated antibody.
Immunogen
ABCA1 transfected HeLa cells.
Conjugate
MaxLight550
Grade
Affinity Purified
Preparation and Storage
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:RPE)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:RPE)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor®488)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor®488)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor®647)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor®647)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:FITC)
Testing Data
(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:FITC)
Related Product Information for anti-ABCA1 antibody
Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABCA subfamily, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.
The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABCA subfamily, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.
Product Categories/Family for anti-ABCA1 antibody
References
1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function. Pflugers Arch.453:569-579.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ATP-binding cassette sub-family A member 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol
Abca1
NCBI Official Synonym Symbols
Abc1; ABC-1
NCBI Protein Information
ATP-binding cassette sub-family A member 1; ATP-binding cassette 1; ATP-binding cassette transporter 1
UniProt Protein Name
ATP-binding cassette sub-family A member 1
Protein Family
UniProt Gene Name
Abca1
UniProt Synonym Gene Names
Abc1; ABC-1
UniProt Entry Name
ABCA1_MOUSE
Similar Products
Product Notes
The ABCA1 abca1 (Catalog #AAA6254348) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1, ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD) (MaxLight 550) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABCA1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunofluorescence (IF). Suitable for use in Flow Cytometry and Immunofluorescence. Not recommended for use in Western Blot. Other applications not tested. Optimal dilutionsto be determined by the researcher. Note: Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ABCA1 abca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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