Mouse ATP-Binding Cassette Sub-Family A Member 1 (ABCA1) ELISA Kit | ABCA1 elisa kit

Mouse ATP-Binding Cassette Sub-Family A Member 1 (ABCA1) ELISA Kit

Cat. Num. AAA9717132

• Gene Names: Abca1; Abc1; ABC-1
• Reactivity: Mouse
• Synonyms: ATP-Binding Cassette Sub-Family A Member 1 (ABCA1); Mouse ATP-Binding Cassette Sub-Family A Member 1 (ABCA1) ELISA Kit; ABCA1; ABC-1; ABC1; CERP; FLJ14958; HDLDT1; MGC164864; MGC165011; TGD; ATP binding cassette transporter 1; ATP-binding cassette 1; ATP-binding cassette transporter A1; ATP-binding cassette; sub-family A membe; ABCA1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse ATP-binding cassette sub-family A member 1 (ABCA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCA1. No significant cross-reactivity or interference between Mouse ABCA1 and analogues was observed.
• Sequence Length: 2261

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse ATP-binding cassette sub-family A member 1 (ABCA1) Standard Curve.)

Related Product Information for ABCA1 elisa kit

Description: This Mouse ATP-binding cassette sub-family A member 1 (ABCA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA1 in samples. An antibody specific for ABCA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: ABCA1 was discovered as the mutation causing Tangier's Disease by several groups in 1998. Gerd Schmitz's group in Germany and Michael Hayden's group in British Columbia were using standard genetics techniques and DNA from family pedigrees to locate the mutation. Richard Lawn's group at CV Therapeutics in Palo Alto, CA used cDNA microarrays, which were relatively new at the time, to assess gene expression profiles from cell lines created from normal and effected individuals. They showed cell lines from patients with Tangier's disease showed differential regulation of the ABCA1 gene. Subsequent sequencing of the gene identified the mutations. This group received an award from the American Heart Association for their discovery.

NCBI and Uniprot Product Information

• NCBI GI #: 90568038
• NCBI GeneID: 11303
• NCBI Accession #: NP_038482.3
• NCBI GenBank Nucleotide #: NP_038482.3
• UniProt Accession #: P41233
• NCBI Official Full Name: ATP-binding cassette sub-family A member 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 1
• NCBI Official Symbol: Abca1
• NCBI Official Synonym Symbols: Abc1; ABC-1
• NCBI Protein Information: ATP-binding cassette sub-family A member 1
• UniProt Protein Name: ATP-binding cassette sub-family A member 1
• Protein Family: ABC transporter A family
• UniProt Gene Name: Abca1
• UniProt Synonym Gene Names: Abc1; ABC-1
• UniProt Entry Name: ABCA1_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]