Mouse Trefoil Factor 2 ELISA Kit | ITF2 elisa kit

Mouse Trefoil Factor 2 ELISA Kit

Cat. Num. AAA722031

• Gene Names: TFF2; SP; SML1
• Reactivity: Mouse
• Synonyms: Trefoil Factor 2; Mouse Trefoil Factor 2 ELISA Kit; ITF2 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ITF2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse ITF2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITF2 elisa kit

Principle of the Assay: ITF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITF2 antibody and an ITF2-HRP conjugate. The assay sample and buffer are incubated together with ITF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITF2 concentration since ITF2 from samples and ITF2-HRP conjugate compete for the anti-ITF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITF2 from the sample, fewer sites are left to bind ITF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITF2 concentration in each sample is interpolated from this standard curve

Product Categories/Family for ITF2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4885629
• NCBI GeneID: 7032
• NCBI Accession #: NP_005414.1
• NCBI GenBank Nucleotide #: NM_005423.4
• Molecular Weight: 14,284 Da
• NCBI Official Full Name: trefoil factor 2
• NCBI Official Synonym Full Names: trefoil factor 2
• NCBI Official Symbol: TFF2
• NCBI Official Synonym Symbols: SP; SML1
• NCBI Protein Information: trefoil factor 2; spasmolysin; spasmolytic protein 1; spasmolytic polypeptide
• UniProt Protein Name: Trefoil factor 2
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF2
• UniProt Synonym Gene Names: SML1; SP
• UniProt Entry Name: TFF2_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF2: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; CXCR4 chemokine receptor binding

Biological Process: negative regulation of inflammatory response; digestion; positive regulation of cell proliferation; calcium-mediated signaling; negative regulation of macrophage activation; regulation of cell migration