ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 2B4 protein, mFc-His tagged protein can bind Rabbit anti-2B4 monoclonal antibody (clone: DM70) in a linear range of 1-100 ng/ml.)
Rabbit anti-Human 2B4 Monoclonal Antibody | anti-2B4 antibody
Anti-2B4 antibody (DM70), Rabbit mAb
FC/FACS: 1:100
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 2B4 protein, mFc-His tagged protein can bind Rabbit anti-2B4 monoclonal antibody (clone: DM70) in a linear range of 1-100 ng/ml.)
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human 2B4 protein, mFc-His tagged protein can bind Rabbit anti-2B4 monoclonal antibody (clone: DM70) in a linear range of 1-100 ng/ml.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1ug/ml (clone: DM70) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1ug/ml (clone: DM70) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM70) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM70) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Uniprot Description
CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: signal transduction; blood coagulation; leukocyte migration
Disease: Rheumatoid Arthritis
Research Articles on 2B4
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Product Notes
The 2B4 cd244 (Catalog #AAA188202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-2B4 antibody (DM70), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 2B4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the 2B4 cd244 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "2B4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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