Western Blot (WB)
(Western blot analysis of whole cell lysates probed with 14-3-3 beta/alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 82.1 second exposure. Arrow points to 14-3-3 beta/alpha (molecular weight 28 kDa))
Mouse 14-3-3 Beta/Alpha Monoclonal Antibody | anti-YWHAB antibody
14-3-3 Beta/Alpha Antibody with Control Lysate
Western Blotting: Detects a band of approximately 28 kDa in MCF7 cell lysates.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with 14-3-3 beta/alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 82.1 second exposure. Arrow points to 14-3-3 beta/alpha (molecular weight 28 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with 14-3-3 beta/alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 82.1 second exposure. Arrow points to 14-3-3 beta/alpha (molecular weight 28 kDa))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Research Articles on YWHAB
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Product Notes
The YWHAB ywhab (Catalog #AAA225457) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 Beta/Alpha Antibody with Control Lysate reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 Beta/Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 28 kDa in MCF7 cell lysates. Researchers should empirically determine the suitability of the YWHAB ywhab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 Beta/Alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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