Western Blot (WB)
(Western blot analysis of whole cell lysates probed with kinesin family member 2C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 42 second exposure. Arrow points to kinesin family member 2C (molecular weight 81 kDa))
Mouse anti-Human Kinesin Family Member 2C Monoclonal Antibody | anti-KIF2C antibody
Kinesin Family Member 2C Antibody with Control Lysate
Gene Names
KIF2C; MCAK; CT139; KNSL6
Reactivity
Human
Applications
Western Blot
Purity
Purified by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Kinesin Family Member 2C; Monoclonal Antibody; Kinesin Family Member 2C Antibody with Control Lysate; Mouse anti KINESIN FAMILY MEMBER 2C; Kinesin Family Member 2C Antibody; anti-KIF2C antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CPTC20
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Sequence Length
725
Applicable Applications for anti-KIF2C antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Western Blotting: Detects a band of approximately 81 kDa in Jurkat cell lysates.
Western Blotting: Detects a band of approximately 81 kDa in Jurkat cell lysates.
Immunogen
Recombinant protein of human kinesin family member 2C
Lysate Composition
400ug Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with kinesin family member 2C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 42 second exposure. Arrow points to kinesin family member 2C (molecular weight 81 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with kinesin family member 2C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 42 second exposure. Arrow points to kinesin family member 2C (molecular weight 81 kDa))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
81 kDa
NCBI Official Full Name
Kinesin-like protein KIF2C
NCBI Official Synonym Full Names
kinesin family member 2C
NCBI Official Symbol
KIF2C
NCBI Official Synonym Symbols
MCAK; CT139; KNSL6
NCBI Protein Information
kinesin-like protein KIF2C
UniProt Protein Name
Kinesin-like protein KIF2C
Protein Family
UniProt Gene Name
KIF2C
UniProt Synonym Gene Names
KNSL6; MCAK
NCBI Description
This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).
Research Articles on KIF2C
Similar Products
Product Notes
The KIF2C kif2c (Catalog #AAA225520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Kinesin Family Member 2C Antibody with Control Lysate reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kinesin Family Member 2C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 81 kDa in Jurkat cell lysates. Researchers should empirically determine the suitability of the KIF2C kif2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kinesin Family Member 2C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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