Western Blot (WB)
(Western blot analysis of whole cell lysates probed with actin alpha 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 31 second exposure. Arrow points to actin alpha 2 (molecular weight 42 kDa))
Mouse Actin Alpha 2 Monoclonal Antibody | anti-ACTA2 antibody
Actin Alpha 2 Antibody with Control Lysate
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Actin Alpha 2; Monoclonal Antibody; Actin Alpha 2 Antibody with Control Lysate; Mouse anti ACTIN ALPHA 2; Actin Alpha 2 Antibody; anti-ACTA2 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Sequence Length
377
Applicable Applications for anti-ACTA2 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Western Blotting: Detects a band of approximately 42 kDa in HeLa cell lysate.
Western Blotting: Detects a band of approximately 42 kDa in HeLa cell lysate.
Immunogen
E Coli derived recombinant protein corresponding to amino acids 1-377 of human actin alpha 2
Lysate Composition
400ug HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with actin alpha 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 31 second exposure. Arrow points to actin alpha 2 (molecular weight 42 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with actin alpha 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 31 second exposure. Arrow points to actin alpha 2 (molecular weight 42 kDa))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42 kDa
NCBI Official Full Name
Actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
ACTSA
NCBI Protein Information
actin, aortic smooth muscle
UniProt Protein Name
Actin, aortic smooth muscle
Protein Family
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
Uniprot Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Articles on ACTA2
Similar Products
Product Notes
The ACTA2 acta2 (Catalog #AAA225499) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin Alpha 2 Antibody with Control Lysate reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Alpha 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 42 kDa in HeLa cell lysate. Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Alpha 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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