Typical Testing Data/Standard Curve (for reference only)
Beta-2-Microglobulin (b2M) ELISA Kit | b2M elisa kit
Monkey Beta-2-Microglobulin (b2M) ELISA Kit
Reactivity
Rhesus monkey
Synonyms
Beta-2-Microglobulin (b2M); Monkey Beta-2-Microglobulin (b2M) ELISA Kit; b2M elisa kit
Reactivity
Rhesus monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of b2M. No significant cross-reactivity or interference between b2M and analogues was observed.
Sequence Length
119
Samples
Monkey serum, plasma,urine and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ug/mL
Sensitivity
<0.037ug/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level b2M were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level b2M were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for b2M elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of b2M in monkey serum, plasma,urine and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to b2M. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to b2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain b2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of b2M in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to b2M. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to b2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain b2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of b2M in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,626 Da
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
Protein Family
UniProt Gene Name
B2M
Uniprot Description
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system ().
Research Articles on b2M
Similar Products
Product Notes
The Rhesus monkey b2M b2m (Catalog #AAA456575) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456575 ELISA Kit recognizes Rhesus monkey b2M. It is sometimes possible for the material contained within the vial of "Beta-2-Microglobulin (b2M), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
