Rat Beta-2-Microglobulin (b2M) ELISA Kit | b2M elisa kit

Rat Beta-2-Microglobulin (b2M) ELISA Kit

Cat. Num. AAA2020971

• Reactivity: Rat
• Synonyms: Beta-2-Microglobulin (b2M); Rat Beta-2-Microglobulin (b2M) ELISA Kit; b2M elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-2-Microglobulin (b2M); No significant cross-reactivity or interference between Beta-2-Microglobulin (b2M) and analogues was observed.
• Sequence Length: 119

• Samples: Serum, plasma, urine and other biological fluids
• Assay Type: Competitive
• Detection Range: 246.91-20000pg/mL The standard curve concentrations used for the ELISA's were 20000pg/mL, 6666.67pg/mL, 2222.22pg/mL, 740.74pg/mL, 246.91pg/mL
• Sensitivity: Typically less than 91.2pg/mL.
• Organism Species: Rattus norvegicus (Rat)
• ELISA Type: Competitive
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for b2M elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Beta-2-Microglobulin (b2M) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Beta-2-Microglobulin (b2M) and unlabeled Beta-2-Microglobulin (b2M) (Standards or samples) with the pre-coated antibody specific for Beta-2-Microglobulin (b2M). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-2-Microglobulin (b2M) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-2-Microglobulin (b2M) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4757826
• NCBI GeneID: 567
• NCBI Accession #: NP_004039.1
• NCBI GenBank Nucleotide #: NM_004048.2
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• Molecular Weight: 13,715 Da
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; endoplasmic reticulum lumen; early endosome membrane; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; antigen processing and presentation of peptide antigen via MHC class I; iron ion homeostasis; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic