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Typical Testing Data/Standard Curve (for reference only)

TNFSF13/APRIL elisa kit

Monkey primate TNFSF13/APRIL PicoKine ELISA Kit

Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Synonyms
TNFSF13/APRIL; Monkey primate TNFSF13/APRIL PicoKine ELISA Kit; Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta; Tumor necrosis factor ligand superfamily member 13; TNFSF13; TNFSF13/APRIL elisa kit
Ordering
For Research Use Only!
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant 215 Monkey primate TNFSF13/APRIL
Sequence Length
223
Applicable Applications for TNFSF13/APRIL elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNFSF13/APRIL elisa kit
Principle of the Assay: 215 Monkey primate TNFSF13/APRIL ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate TNFSF13/APRIL has been precoated onto 96-well plates. Standards(Expression system for standard: NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate TNFSF13/APRIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate TNFSF13/APRIL amount of sample captured in plate.

Background: Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation- inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24279 MW
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13 isoform delta
UniProt Protein Name
Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta
UniProt Gene Name
TNFSF13

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Product Notes

The Monkey There is no detectable cross-reactivity with other relevant proteins. TNFSF13/APRIL tnfsf13 (Catalog #AAA1751081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751081 ELISA Kit recognizes Monkey There is no detectable cross-reactivity with other relevant proteins. TNFSF13/APRIL. AAA Biotech's TNFSF13/APRIL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the TNFSF13/APRIL tnfsf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFSF13/APRIL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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