TIMP-3 elisa kit

Monkey primate TIMP-3 PicoKine ELISA Kit

Cat. Num. AAA1751063

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: TIMP-3; Monkey primate TIMP-3 PicoKine ELISA Kit; Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP3; TIMP-3 elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Monkey primate TIMP-3
• Sequence Length: 211

• Applicable Applications for TIMP-3 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP-3 elisa kit

Principle of the Assay: 215 Monkey primate TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate TIMP-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate TIMP-3 amount of sample captured in plate.

Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

Product Categories/Family for TIMP-3 elisa kit

Cell Biology; Apoptosis; Extracellular Signals; Granzymes; Cardiovascular; Angiogenesis; Adhesion/ECM; Matrix Metalloproteinases; TIMP; Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Enzymes; MMP Inhibitors; Neuroscience; Sensory System

NCBI and Uniprot Product Information

• NCBI GI #: 4507513
• NCBI GeneID: 7078
• NCBI Accession #: NP_000353.1
• NCBI GenBank Nucleotide #: NM_000362.4
• UniProt Accession #: P35625; Q5THV4; Q9UC74; Q9UGS2; B2RBY9
• Molecular Weight: 24145 MW
• NCBI Official Full Name: metalloproteinase inhibitor 3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• UniProt Gene Name: TIMP3
• UniProt Synonym Gene Names: TIMP-3

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.