Mouse Macrophage Inflammatory Protein 3 Alpha ELISA Kit | MIP-3alpha elisa kit

Mouse Macrophage Inflammatory Protein 3 Alpha ELISA Kit

Cat. Num. AAA765868

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Mouse
• Synonyms: Macrophage Inflammatory Protein 3 Alpha; Mouse Macrophage Inflammatory Protein 3 Alpha ELISA Kit; MIP-3alpha/exodus-1/LARC/MIP-3 alpha/beta chemokine exodus-1/Beta-chemokine exodus-1/CC chemokine LARC/C-C motif chemokine 20/chemokine (C-C motif) ligand 20/CKb4/exodus-1/LARCLiver and activation-regulated chemokine/MIP-3a/MIP-3-alpha/MIP3AMacrophage inflammatory protein 3 alpha/SCYA20Small-inducible cytokine A20/small inducible cytokine subfamily A (Cys-Cys); member 20/ST38/; MIP-3alpha elisa kit

• Reactivity: Mouse
• Sequence Length: 96

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 7.813-500pg/ml
• Sensitivity: 4.688pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MIP-3alpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse MIP-3alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse MIP-3alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MIP-3alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse MIP-3alpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1790925
• NCBI GeneID: 6364
• Molecular Weight: 10,691 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; protein binding

Biological Process: cell-cell signaling; chemotaxis; defense response to bacterium; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; signal transduction; wound healing