Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha) ELISA Kit | MIP-3alpha elisa kit

Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha) ELISA kit

Cat. Num. AAA269168

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Porcine
• Synonyms: Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha); Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha) ELISA kit; Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3a) ELISA kit; MIP-3alpha elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 96

• Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MIP-3alpha concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-3alpha elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine MIP-3alpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 1790925
• NCBI GeneID: 6364
• Molecular Weight: 10,691 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: wound healing; cell-cell signaling; defense response to bacterium; immune response; signal transduction; positive regulation of interleukin-1 alpha biosynthetic process; inflammatory response; chemotaxis; positive regulation of nitric-oxide synthase biosynthetic process