Western Blot (WB)
(Western blot analysis of EGFR (pY1197) expression in A431 (A), NIH3T3 (B) whole cell lysates.)
Rabbit anti-Human, Mouse EGFR Polyclonal Antibody | anti-EGFR antibody
Anti-EGFR (pY1197) Phospho Antibody
Western Blot (WB)
(Western blot analysis of EGFR (pY1197) expression in A431 (A), NIH3T3 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EGFR (pY1197) expression in A431 (A), NIH3T3 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EGFR (pY1197) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EGFR (pY1197) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.
Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Tumor suppressor; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; EGFR family
Chromosomal Location of Human Ortholog: 7p12
Cellular Component: AP-2 adaptor complex; basolateral plasma membrane; cell surface; cytoplasm; early endosome membrane; endoplasmic reticulum membrane; endosome; endosome membrane; extracellular space; focal adhesion; Golgi membrane; integral to membrane; lipid raft; membrane; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex
Molecular Function: actin filament binding; ATP binding; chromatin binding; double-stranded DNA binding; enzyme binding; epidermal growth factor receptor activity; identical protein binding; MAP kinase kinase kinase activity; nitric-oxide synthase regulator activity; protein binding; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding
Biological Process: activation of MAPKK activity; axon guidance; calcium-dependent phospholipase A2 activation; cell proliferation; cell surface receptor linked signal transduction; cell-cell adhesion; cerebral cortex cell migration; digestive tract morphogenesis; embryonic placenta development; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; hair follicle development; innate immune response; insulin receptor signaling pathway; learning and/or memory; MAPKKK cascade; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of protein catabolic process; nerve growth factor receptor signaling pathway; ossification; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA repair; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein insertion into membrane; Ras protein signal transduction; regulation of nitric-oxide synthase activity; regulation of peptidyl-tyrosine phosphorylation; response to stress; salivary gland morphogenesis; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway
Disease: Lung Cancer
Research Articles on EGFR
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Product Notes
The EGFR egfr (Catalog #AAA8242010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EGFR (pY1197) Phospho Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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