Human MMP-3 ELISA Kit | MMP3 elisa kit

Human MMP-3 PicoKine ELISA Kit

Cat. Num. AAA175977

• Gene Names: MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• Reactivity: Human
• Synonyms: MMP-3; Human MMP-3 PicoKine ELISA Kit; Stromelysin-1; matrix metallopeptidase 3 (stromelysin 1; progelatinase); CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase 3 preproprotein; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP3; MMP3_HUMAN; Progelatinase; Proteoglycanase; SL 1; SL-1; SL1; STMY; STMY1; STR1; Stromelisin 1; Stromelysin 1; Stromelysin 1 progelatinase; Transin 1; Transin-1; MMP3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human total MMP-3
• Sequence Length: 477

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human MMP-3 PicoKine ELISA Kit standard curve )

Related Product Information for MMP3 elisa kit

Principle of the assay: human MMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-3 has been precoated onto 96-well plates. Standards (NSO,Y18-C477) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-3 amount of sample captured in plate.
Background: Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 116857
• NCBI GeneID: 4314
• UniProt Accession #: P08254; Q3B7S0; Q6GRF8; B2R8B8
• Molecular Weight: 53,977 Da
• NCBI Official Full Name: Stromelysin-1
• NCBI Official Synonym Full Names: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• NCBI Official Symbol: MMP3
• NCBI Official Synonym Symbols: SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• NCBI Protein Information: stromelysin-1; transin-1; proteoglycanase; matrix metalloproteinase-3; matrix metalloproteinase 3 (stromelysin 1, progelatinase)
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: MMP3
• UniProt Synonym Gene Names: STMY1; SL-1; MMP-3
• UniProt Entry Name: MMP3_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.

Protein type: Secreted; EC 3.4.24.17; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; mitochondrion; extracellular region; cytosol

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration

Disease: Coronary Heart Disease, Susceptibility To, 6