Human MMP-1 ELISA Kit | MMP1 elisa kit

Human MMP-1 PicoKine ELISA Kit

Cat. Num. AAA175893

• Gene Names: MMP1; CLG; CLGN
• Reactivity: Human
• Synonyms: MMP-1; Human MMP-1 PicoKine ELISA Kit; Interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); 27 kDa interstitial collagenase; CLG; CLGN; Fibroblast collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP1; MMP1_HUMAN; MMP1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MMP1
• Sequence Length: 403

• Samples: Cell culture supernates, serum and plasma(heparin)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP1 elisa kit

Principle of the Assay: The Picokine™ Human MMP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MMP1 with a 96-well strip plate that is pre-coated with antibody specific for MMP1. The detection antibody is a biotinylated antibody specific for MMP1. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MMP1 with immunogen: Expression system for standard: NSO; Immunogen sequence: F20-N469. The kit is analytically validated with ready to use reagents. To measure Human MMP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MMP1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MMP1 in the sample.

Background/Introduction: Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smokinginduced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GeneID: 4312
• UniProt Accession #: B4DN15; P03956
• Molecular Weight: 46,266 Da
• NCBI Official Synonym Full Names: matrix metallopeptidase 1 (interstitial collagenase)
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1
• UniProt Protein Name: cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7)
• Protein Family: Interstitial collagenase
• UniProt Entry Name: B4DN15_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.7; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; proteolysis; blood coagulation; leukocyte migration

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive