Mouse anti-Human MMP-1 Monoclonal Antibody | anti-MMP-1 antibody

Mouse anti Human MMP-1

Cat. Num. AAA226205

• Gene Names: MMP1; CLG; CLGN
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: MMP-1; Monoclonal Antibody; Mouse anti Human MMP-1; MMP-1 antibody | F08-2F1; anti-MMP-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: F08-2F1
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-MMP-1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant human partial length of MMP-1 (Phe100-Thr274)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human MMP-1 using Mouse anti Human MMP-1 (MBS226205) as a capture reagent and biotinylated Mouse anti Human MMP-1 as a detection reagent with purified recombinant MMP-1 as the antigen. Detection was performed using HR)

Related Product Information for anti-MMP-1 antibody

Mouse anti Human MMP-1 antibody, clone F08-2F1, recognizes matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase. MMP-1 is a 45kDa protein in its active form and is a member of the metzincins superfamily of proteases. It is produced by various cells and tissues including the endothelium, fibroblasts, smooth muscle cells, vascular adventitia and platelets (Cui et al. 2017).

MMP-1 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the pro-MMP-1. This is then further cleaved by proteolytic enzymes to create the active form. Like other MMP’s, MMP-1 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-1 degrades the collagen proteins type I, II, III, VII, VIII, X and gelatin. It is also able to target other ECM substrates including aggrecan, nidogen and perlecan (Cui et al. 2017).

MMP1 expression levels are low in most cells under physiological conditions, but are upregulated during inflammatory conditions and autoimmune diseases (Mittal et al. 2016). For example a single nucleotide polymorphism of MMP-1 at the promoter region has been associated with an increased risk of sudden deafness linked to circulatory disturbance and inflammation (Nam et al. 2011).

The purified Mouse anti Human MMP-1 antibody, clone F08-2F1 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human MMP-1 antibody, clone F09-3C7 as the detection antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4505215
• NCBI GeneID: 4312
• NCBI Accession #: NP_002412.1
• NCBI GenBank Nucleotide #: NM_002421.3
• UniProt Accession #: P03956; P08156
• Molecular Weight: 54,007 Da
• NCBI Official Full Name: interstitial collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 1 (interstitial collagenase)
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1
• UniProt Protein Name: Interstitial collagenase
• UniProt Gene Name: MMP1
• UniProt Synonym Gene Names: CLG; MMP-1
• UniProt Entry Name: MMP1_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted, signal peptide; EC 3.4.24.7; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: positive regulation of protein oligomerization; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; blood coagulation; proteolysis; leukocyte migration

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive