Mouse MMP-1 Monoclonal Antibody | anti-MMP-1 antibody

MOUSE ANTI HUMAN MMP-1

Cat. Num. AAA217371

• Gene Names: MMP1; CLG; CLGN
• Applications: Immunohistochemistry, Western Blot
• Synonyms: MMP-1; Monoclonal Antibody; MOUSE ANTI HUMAN MMP-1; anti-MMP-1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3B6
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 469

• Applicable Applications for anti-MMP-1 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 37kDa ; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Histology Positive Control Tissue: Colon carcinoma; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of paraffin embedded normal human colon with Mouse anti Human MMP-1 (MBS217372) after citrate retrieval)

Related Product Information for anti-MMP-1 antibody

Mouse anti Human MMP-1 antibody, clone 3B6 recognizes human matrix metalloproteinase 1 (MMP-1), also known as interstitial or fibroblast collagenase, a 469 amino acid, in the pro-peptide form, zinc-dependent endopeptidase responsible for degrading the extracellular matrix. MMPs are also involved in cell proliferation, migration, differentiation and apoptosis (Chen et al. 2013). Most MMP's are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active (Nagase et al. 1992). MMP expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis (Murray et al. 1996). MMP-1 is one of the collagenases, capable of degrading collagens I, II and III, all main components of the interstitial stroma (Robichaud et al. 2011). MMP-1 is overexpressed in invasive melanoma, colorectal and esophageal cancers (Langenskiöld et al. 2013). MMP-1 has also been implicated in arthritis and may influence atherosclerotic lesion formation (Nikkari et al. 1995). Additionally, MMP-1 has been implicated in the repair processes of the heart after myocardial infarction (Yarbrough et al. 2003). Mouse anti Human MMP-1 has been used successfully for the detection of MMP-1 in gastric cancer by both Western blotting and immunohistochemistry (Murray et al. 1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4505215
• NCBI GeneID: 4312
• NCBI Accession #: NP_002412.1
• NCBI GenBank Nucleotide #: NM_002421.3
• UniProt Accession #: P03956; P08156
• Molecular Weight: 54,007 Da
• NCBI Official Full Name: interstitial collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 1
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase; fibroblast collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1
• UniProt Protein Name: Interstitial collagenase
• UniProt Gene Name: MMP1
• UniProt Synonym Gene Names: CLG; MMP-1
• UniProt Entry Name: MMP1_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.7; Secreted, signal peptide; Secreted; Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; proteolysis; blood coagulation; leukocyte migration

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive