Human Aggrecan core protein ELISA Kit | ACAN elisa kit

Human Aggrecan core protein ELISA Kit

Cat. Num. AAA762254

• Gene Names: ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• Reactivity: Human
• Synonyms: Aggrecan core protein; Human Aggrecan core protein ELISA Kit; ACAN/Aggrecan core protein/Cartilage-specific proteoglycan core protein/CSPCP/Chondroitin sulfate proteoglycan core protein 1/Chondroitin sulfate proteoglycan 1/AGC1/CSPG1/MSK16/AGCAN/CSPGCP/SEDK; ACAN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ABO. No significant cross-reactivity or interference between ABO and analogues was observed.
• Sequence Length: 2530

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ABO were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ABO were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACAN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ABO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ABO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ABO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ABO can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 256017257
• NCBI GeneID: 176
• NCBI Accession #: NP_037359.3
• NCBI GenBank Nucleotide #: NM_013227.3
• Molecular Weight: 239,235 Da
• NCBI Official Full Name: aggrecan core protein isoform 2
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• NCBI Protein Information: aggrecan core protein
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1
• UniProt Entry Name: PGCA_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; extracellular region; Golgi lumen; lysosomal lumen; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; extracellular matrix structural constituent; hyaluronic acid binding; metal ion binding; protein binding

Biological Process: carbohydrate metabolic process; cell adhesion; central nervous system development; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; keratan sulfate catabolic process; keratan sulfate metabolic process; proteolysis; skeletal development

Disease: Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type; Spondyloepiphyseal Dysplasia, Kimberley Type