SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein gel 13% SDS-PAGE Coomassie staining MW: 19.8 kDa Purity: >60%)
MLLT3/AF9 recombinant protein
Recombinant MLLT3/AF9 (1-138) protein
Gene Names
MLLT3; AF9; YEATS3
Synonyms
MLLT3/AF9; Recombinant MLLT3/AF9 (1-138) protein; MLLT3/AF9 recombinant protein
Host
E Coli
Form/Format
Recombinant MLLT3/AF9 (1-138) protein is supplied in 25mM Tris-HCl pH7.4, 500mM NaCl, 2mM EDTA, 2mM DTT, 10% glycerol, 0.5mM TCEP.
Protein Species
Human
Tag
His-Tag
Notes
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. Binding Assay for Recombinant MLLT3/AF9 (1-138) protein: 0.25nmol biotinylated H3K9ac peptide was incubated with 0.25nmol MLLT3/AF9 (1-138) protein in binding buffer containing 20mM Tris-HCl pH 8.0, 150mM NaCl, 5% glycerol, 0.1% Triton X-100. Peptide-protein compounds were incubated with streptavidin beads for 4 hours at 4 degree C, and pull-down products were run on a 10% SDS-PAGE gel, stained with Coomassie Blue. (Single MLLT3/AF9 (1-138) protein was used as negative control). * indicates streptavidin.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein gel 13% SDS-PAGE Coomassie staining MW: 19.8 kDa Purity: >60%)
SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein gel 13% SDS-PAGE Coomassie staining MW: 19.8 kDa Purity: >60%)
SDS-Page
(Binding Assay for Recombinant MLLT3 / AF9 protein 0.25 nmol biotinylated H3K9ac peptide was incubated with 0.25 nmol MLLT3 / AF9 (1-138) protein in binding buffer. Peptide-protein compounds were incubated with streptavidin beads and pull-down products were run on SDS-PAGE gel, stained with Coomassie Blue. (Single MLLT3 / AF9 protein used as negative control). * indicates Streptavidin.)
SDS-Page
(Binding Assay for Recombinant MLLT3 / AF9 protein 0.25 nmol biotinylated H3K9ac peptide was incubated with 0.25 nmol MLLT3 / AF9 (1-138) protein in binding buffer. Peptide-protein compounds were incubated with streptavidin beads and pull-down products were run on SDS-PAGE gel, stained with Coomassie Blue. (Single MLLT3 / AF9 protein used as negative control). * indicates Streptavidin.)
Related Product Information for MLLT3/AF9 recombinant protein
Short Description: Recombinant MLLT3/AF9 (1-138) protein was expressed in E Coli as the full-length protein (accession number NP_004520.2) with an N-terminal 6xHis-tag. The molecular weight of the protein is 19.8 kDa. It is suitable for use in in binding assays, inhibitor screening, and selectivity profiling.
Background: MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein), also known as YEATS3 or AF9, is a chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. MLLT3 recognizes and binds histone H3 crotonylated at Lys-9 (H3K9cr), and with slightly lower affinity histone H3 crotonylated at Lys-18 (H3K18cr). It also recognizes and binds histone H3 acetylated at Lys-9 (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.
Background: MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein), also known as YEATS3 or AF9, is a chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. MLLT3 recognizes and binds histone H3 crotonylated at Lys-9 (H3K9cr), and with slightly lower affinity histone H3 crotonylated at Lys-18 (H3K18cr). It also recognizes and binds histone H3 acetylated at Lys-9 (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.
Product Categories/Family for MLLT3/AF9 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,106 Da
NCBI Official Full Name
protein AF-9 isoform a
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
NCBI Official Symbol
MLLT3
NCBI Official Synonym Symbols
AF9; YEATS3
NCBI Protein Information
protein AF-9; YEATS domain-containing protein 3; ALL1-fused gene from chromosome 9 protein; myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3
UniProt Protein Name
Protein AF-9
UniProt Gene Name
MLLT3
UniProt Synonym Gene Names
AF9; YEATS3
UniProt Entry Name
AF9_HUMAN
Uniprot Description
MLLT3: A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
Protein type: Cell development/differentiation; Oncoprotein
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: transcription elongation factor complex; nucleus
Molecular Function: protein binding
Biological Process: anterior/posterior pattern formation; segment specification; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on MLLT3/AF9
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Product Notes
The MLLT3/AF9 mllt3 (Catalog #AAA389181) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MLLT3/AF9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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